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antagonist su5402  (MedChemExpress)


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    Structured Review

    MedChemExpress antagonist su5402
    FGF3 enhances the expression of repellent molecule Slit1 . ( A , A’ ) Western blot analyses showed that, compared with blank controls, FGF3 (300 ng/mL) in the culture media significantly increased the expression of Slit1 (n = 6). ( B , B’ ) In contrast, the FGFR inhibitor <t>SU5402</t> (20 µM) reduced the expression of the repellent factor Slit1 (n = 6). ( C – H ) Enhanced immunofluorescence staining of DAPI (blue) and Slit1 (green) shows FGF3 upregulates Slit1 expression: explants of E4 diencephalon were cultured either for two days alone ( C , D ), with FGF3 ( E , F ), or with FGF3+SU5402 in the media ( G , H ). ( I ) Quantification of fluorescent signal from immuno-labelled Slit1 per volume of the thalamic explant. ( J ) Top GO items of RNA-seq were shown according to the gene counts. ( K ) Heatmap visualizes mRNA expression patterns of relative genes across different groups (controls and FGF3-treated). ( L ) Bar graphs for specific genes at mRNA transcription level ( Vglut2 , Mki67 , Pcna ), comparing their expression between controls and FGF3-treated explants. ns: Not-significant; FPKM, fragments per kilobase of transcript per million mapped reads; GO, gene ontology; **: p < 0.01; ***: p < 0.001; Scale bars = 40 µm.
    Antagonist Su5402, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antagonist+su5402/SU+5402/pmc12347462-162-16-18
    Average 94 stars, based on 23 article reviews
    antagonist su5402 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Direct and Indirect Downstream Pathways That Regulate Repulsive Guidance Effects of FGF3 on Developing Thalamocortical Axons"

    Article Title: Direct and Indirect Downstream Pathways That Regulate Repulsive Guidance Effects of FGF3 on Developing Thalamocortical Axons

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms26157361

    FGF3 enhances the expression of repellent molecule Slit1 . ( A , A’ ) Western blot analyses showed that, compared with blank controls, FGF3 (300 ng/mL) in the culture media significantly increased the expression of Slit1 (n = 6). ( B , B’ ) In contrast, the FGFR inhibitor SU5402 (20 µM) reduced the expression of the repellent factor Slit1 (n = 6). ( C – H ) Enhanced immunofluorescence staining of DAPI (blue) and Slit1 (green) shows FGF3 upregulates Slit1 expression: explants of E4 diencephalon were cultured either for two days alone ( C , D ), with FGF3 ( E , F ), or with FGF3+SU5402 in the media ( G , H ). ( I ) Quantification of fluorescent signal from immuno-labelled Slit1 per volume of the thalamic explant. ( J ) Top GO items of RNA-seq were shown according to the gene counts. ( K ) Heatmap visualizes mRNA expression patterns of relative genes across different groups (controls and FGF3-treated). ( L ) Bar graphs for specific genes at mRNA transcription level ( Vglut2 , Mki67 , Pcna ), comparing their expression between controls and FGF3-treated explants. ns: Not-significant; FPKM, fragments per kilobase of transcript per million mapped reads; GO, gene ontology; **: p < 0.01; ***: p < 0.001; Scale bars = 40 µm.
    Figure Legend Snippet: FGF3 enhances the expression of repellent molecule Slit1 . ( A , A’ ) Western blot analyses showed that, compared with blank controls, FGF3 (300 ng/mL) in the culture media significantly increased the expression of Slit1 (n = 6). ( B , B’ ) In contrast, the FGFR inhibitor SU5402 (20 µM) reduced the expression of the repellent factor Slit1 (n = 6). ( C – H ) Enhanced immunofluorescence staining of DAPI (blue) and Slit1 (green) shows FGF3 upregulates Slit1 expression: explants of E4 diencephalon were cultured either for two days alone ( C , D ), with FGF3 ( E , F ), or with FGF3+SU5402 in the media ( G , H ). ( I ) Quantification of fluorescent signal from immuno-labelled Slit1 per volume of the thalamic explant. ( J ) Top GO items of RNA-seq were shown according to the gene counts. ( K ) Heatmap visualizes mRNA expression patterns of relative genes across different groups (controls and FGF3-treated). ( L ) Bar graphs for specific genes at mRNA transcription level ( Vglut2 , Mki67 , Pcna ), comparing their expression between controls and FGF3-treated explants. ns: Not-significant; FPKM, fragments per kilobase of transcript per million mapped reads; GO, gene ontology; **: p < 0.01; ***: p < 0.001; Scale bars = 40 µm.

    Techniques Used: Expressing, Western Blot, Immunofluorescence, Staining, Cell Culture, RNA Sequencing

    Related Articles

    Expressing:

    Article Title: Direct and Indirect Downstream Pathways That Regulate Repulsive Guidance Effects of FGF3 on Developing Thalamocortical Axons
    Article Snippet: Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 °C prior to culture.Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 °C prior to culture.. FGF3 protein was used, based on standards, at concentrations of 300 ng/mL or 500 ng/mL; its antagonist SU5402 (MedChemExpress, Monmouth Junction, NJ, USA) was used at 20 μM.. Slit1 (R&D) protein was used at concentrations of 200 ng/mL [ ].Slit1 (R&D) protein was used at concentrations of 200 ng/mL [ ].

    Article Title: Direct and Indirect Downstream Pathways That Regulate Repulsive Guidance Effects of FGF3 on Developing Thalamocortical Axons.
    Article Snippet: Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 ◦C prior to culture.Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 ◦C prior to culture.. FGF3 protein was used, based on standards, at concentrations of 300 ng/mL or 500 ng/mL; its antagonist SU5402 (MedChemExpress, Monmouth Junction, NJ, USA) was used at 20 μM.. Slit1 (R&D) protein was used at concentrations of 200 ng/mL [49].Slit1 (R&D) protein was used at concentrations of 200 ng/mL [49].

    Western Blot:

    Article Title: Direct and Indirect Downstream Pathways That Regulate Repulsive Guidance Effects of FGF3 on Developing Thalamocortical Axons
    Article Snippet: Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 °C prior to culture.Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 °C prior to culture.. FGF3 protein was used, based on standards, at concentrations of 300 ng/mL or 500 ng/mL; its antagonist SU5402 (MedChemExpress, Monmouth Junction, NJ, USA) was used at 20 μM.. Slit1 (R&D) protein was used at concentrations of 200 ng/mL [ ].Slit1 (R&D) protein was used at concentrations of 200 ng/mL [ ].

    Article Title: Direct and Indirect Downstream Pathways That Regulate Repulsive Guidance Effects of FGF3 on Developing Thalamocortical Axons.
    Article Snippet: Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 ◦C prior to culture.Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 ◦C prior to culture.. FGF3 protein was used, based on standards, at concentrations of 300 ng/mL or 500 ng/mL; its antagonist SU5402 (MedChemExpress, Monmouth Junction, NJ, USA) was used at 20 μM.. Slit1 (R&D) protein was used at concentrations of 200 ng/mL [49].Slit1 (R&D) protein was used at concentrations of 200 ng/mL [49].

    Immunofluorescence:

    Article Title: Direct and Indirect Downstream Pathways That Regulate Repulsive Guidance Effects of FGF3 on Developing Thalamocortical Axons
    Article Snippet: Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 °C prior to culture.Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 °C prior to culture.. FGF3 protein was used, based on standards, at concentrations of 300 ng/mL or 500 ng/mL; its antagonist SU5402 (MedChemExpress, Monmouth Junction, NJ, USA) was used at 20 μM.. Slit1 (R&D) protein was used at concentrations of 200 ng/mL [ ].Slit1 (R&D) protein was used at concentrations of 200 ng/mL [ ].

    Article Title: Direct and Indirect Downstream Pathways That Regulate Repulsive Guidance Effects of FGF3 on Developing Thalamocortical Axons.
    Article Snippet: Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 ◦C prior to culture.Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 ◦C prior to culture.. FGF3 protein was used, based on standards, at concentrations of 300 ng/mL or 500 ng/mL; its antagonist SU5402 (MedChemExpress, Monmouth Junction, NJ, USA) was used at 20 μM.. Slit1 (R&D) protein was used at concentrations of 200 ng/mL [49].Slit1 (R&D) protein was used at concentrations of 200 ng/mL [49].

    Staining:

    Article Title: Direct and Indirect Downstream Pathways That Regulate Repulsive Guidance Effects of FGF3 on Developing Thalamocortical Axons
    Article Snippet: Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 °C prior to culture.Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 °C prior to culture.. FGF3 protein was used, based on standards, at concentrations of 300 ng/mL or 500 ng/mL; its antagonist SU5402 (MedChemExpress, Monmouth Junction, NJ, USA) was used at 20 μM.. Slit1 (R&D) protein was used at concentrations of 200 ng/mL [ ].Slit1 (R&D) protein was used at concentrations of 200 ng/mL [ ].

    Article Title: Direct and Indirect Downstream Pathways That Regulate Repulsive Guidance Effects of FGF3 on Developing Thalamocortical Axons.
    Article Snippet: Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 ◦C prior to culture.Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 ◦C prior to culture.. FGF3 protein was used, based on standards, at concentrations of 300 ng/mL or 500 ng/mL; its antagonist SU5402 (MedChemExpress, Monmouth Junction, NJ, USA) was used at 20 μM.. Slit1 (R&D) protein was used at concentrations of 200 ng/mL [49].Slit1 (R&D) protein was used at concentrations of 200 ng/mL [49].

    Cell Culture:

    Article Title: Direct and Indirect Downstream Pathways That Regulate Repulsive Guidance Effects of FGF3 on Developing Thalamocortical Axons
    Article Snippet: Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 °C prior to culture.Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 °C prior to culture.. FGF3 protein was used, based on standards, at concentrations of 300 ng/mL or 500 ng/mL; its antagonist SU5402 (MedChemExpress, Monmouth Junction, NJ, USA) was used at 20 μM.. Slit1 (R&D) protein was used at concentrations of 200 ng/mL [ ].Slit1 (R&D) protein was used at concentrations of 200 ng/mL [ ].

    Article Title: Direct and Indirect Downstream Pathways That Regulate Repulsive Guidance Effects of FGF3 on Developing Thalamocortical Axons.
    Article Snippet: Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 ◦C prior to culture.Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 ◦C prior to culture.. FGF3 protein was used, based on standards, at concentrations of 300 ng/mL or 500 ng/mL; its antagonist SU5402 (MedChemExpress, Monmouth Junction, NJ, USA) was used at 20 μM.. Slit1 (R&D) protein was used at concentrations of 200 ng/mL [49].Slit1 (R&D) protein was used at concentrations of 200 ng/mL [49].

    RNA Sequencing:

    Article Title: Direct and Indirect Downstream Pathways That Regulate Repulsive Guidance Effects of FGF3 on Developing Thalamocortical Axons
    Article Snippet: Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 °C prior to culture.Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 °C prior to culture.. FGF3 protein was used, based on standards, at concentrations of 300 ng/mL or 500 ng/mL; its antagonist SU5402 (MedChemExpress, Monmouth Junction, NJ, USA) was used at 20 μM.. Slit1 (R&D) protein was used at concentrations of 200 ng/mL [ ].Slit1 (R&D) protein was used at concentrations of 200 ng/mL [ ].

    Article Title: Direct and Indirect Downstream Pathways That Regulate Repulsive Guidance Effects of FGF3 on Developing Thalamocortical Axons.
    Article Snippet: Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 ◦C prior to culture.Affi-Gel Blue gel beads (BioRad #153-7302, Hercules, CA, USA) were washed 3 times in PBS and then soaked in FGF3 protein (R&D) overnight at 4 ◦C prior to culture.. FGF3 protein was used, based on standards, at concentrations of 300 ng/mL or 500 ng/mL; its antagonist SU5402 (MedChemExpress, Monmouth Junction, NJ, USA) was used at 20 μM.. Slit1 (R&D) protein was used at concentrations of 200 ng/mL [49].Slit1 (R&D) protein was used at concentrations of 200 ng/mL [49].



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    Image Search Results


    FGF3 enhances the expression of repellent molecule Slit1 . ( A , A’ ) Western blot analyses showed that, compared with blank controls, FGF3 (300 ng/mL) in the culture media significantly increased the expression of Slit1 (n = 6). ( B , B’ ) In contrast, the FGFR inhibitor SU5402 (20 µM) reduced the expression of the repellent factor Slit1 (n = 6). ( C – H ) Enhanced immunofluorescence staining of DAPI (blue) and Slit1 (green) shows FGF3 upregulates Slit1 expression: explants of E4 diencephalon were cultured either for two days alone ( C , D ), with FGF3 ( E , F ), or with FGF3+SU5402 in the media ( G , H ). ( I ) Quantification of fluorescent signal from immuno-labelled Slit1 per volume of the thalamic explant. ( J ) Top GO items of RNA-seq were shown according to the gene counts. ( K ) Heatmap visualizes mRNA expression patterns of relative genes across different groups (controls and FGF3-treated). ( L ) Bar graphs for specific genes at mRNA transcription level ( Vglut2 , Mki67 , Pcna ), comparing their expression between controls and FGF3-treated explants. ns: Not-significant; FPKM, fragments per kilobase of transcript per million mapped reads; GO, gene ontology; **: p < 0.01; ***: p < 0.001; Scale bars = 40 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Direct and Indirect Downstream Pathways That Regulate Repulsive Guidance Effects of FGF3 on Developing Thalamocortical Axons

    doi: 10.3390/ijms26157361

    Figure Lengend Snippet: FGF3 enhances the expression of repellent molecule Slit1 . ( A , A’ ) Western blot analyses showed that, compared with blank controls, FGF3 (300 ng/mL) in the culture media significantly increased the expression of Slit1 (n = 6). ( B , B’ ) In contrast, the FGFR inhibitor SU5402 (20 µM) reduced the expression of the repellent factor Slit1 (n = 6). ( C – H ) Enhanced immunofluorescence staining of DAPI (blue) and Slit1 (green) shows FGF3 upregulates Slit1 expression: explants of E4 diencephalon were cultured either for two days alone ( C , D ), with FGF3 ( E , F ), or with FGF3+SU5402 in the media ( G , H ). ( I ) Quantification of fluorescent signal from immuno-labelled Slit1 per volume of the thalamic explant. ( J ) Top GO items of RNA-seq were shown according to the gene counts. ( K ) Heatmap visualizes mRNA expression patterns of relative genes across different groups (controls and FGF3-treated). ( L ) Bar graphs for specific genes at mRNA transcription level ( Vglut2 , Mki67 , Pcna ), comparing their expression between controls and FGF3-treated explants. ns: Not-significant; FPKM, fragments per kilobase of transcript per million mapped reads; GO, gene ontology; **: p < 0.01; ***: p < 0.001; Scale bars = 40 µm.

    Article Snippet: FGF3 protein was used, based on standards, at concentrations of 300 ng/mL or 500 ng/mL; its antagonist SU5402 (MedChemExpress, Monmouth Junction, NJ, USA) was used at 20 μM.

    Techniques: Expressing, Western Blot, Immunofluorescence, Staining, Cell Culture, RNA Sequencing

    Wild-type endothelial cells were treated with SU5402 (an inhibitor of FGF signaling) and then serum starved for 16 h. Brightfield images are shown of cells grown without pretreatment with 30 μM SU5402 ( A ) or with pretreatment ( B ) before serum starvation. ( C ) Western blot analysis of cleaved PARP in wild-type endothelial cells pretreated (+) or not (−) with SU5402 and ( D ) absence of cleaved PARP in wild-type endothelial cells pretreated for 16 h with 10 μM inhibitor XXI and then serum starved for an additional 16 h. ( E ) Western blotting shows accumulation of the N-cadherin C-terminal fragment (CTF) following treatment with γ-secretase inhibitor XXI. Scale bar in A, 25 μm.

    Journal: Scientific Reports

    Article Title: Fibroblast growth factor rescues brain endothelial cells lacking presenilin 1 from apoptotic cell death following serum starvation

    doi: 10.1038/srep30267

    Figure Lengend Snippet: Wild-type endothelial cells were treated with SU5402 (an inhibitor of FGF signaling) and then serum starved for 16 h. Brightfield images are shown of cells grown without pretreatment with 30 μM SU5402 ( A ) or with pretreatment ( B ) before serum starvation. ( C ) Western blot analysis of cleaved PARP in wild-type endothelial cells pretreated (+) or not (−) with SU5402 and ( D ) absence of cleaved PARP in wild-type endothelial cells pretreated for 16 h with 10 μM inhibitor XXI and then serum starved for an additional 16 h. ( E ) Western blotting shows accumulation of the N-cadherin C-terminal fragment (CTF) following treatment with γ-secretase inhibitor XXI. Scale bar in A, 25 μm.

    Article Snippet: FGF1, FGF2, and VEGF (R&D Systems, Minneapolis, MN) were added individually at 25 ng/ml in DMEM-BSA-heparin media along with the γ-secretase inhibitor XXI (compound E, 10 μm, EMD Millipore, Billerica, MA) or the FGFR antagonist SU5402 (10 μm, Santa Cruz Biotechnology, Santa Cruz, CA).

    Techniques: Western Blot